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Development of immunometric test for detection of reconstituted (dry) milk adulteration of fresh milk

The adulteration of fresh milk by dry milk (reconstituted from powder) is a common problem in milk industry. The kit is based on monoclonal antibodies that recognize heat-sensitive neoepitope (neoantigen) of milk serum proteins that appear during heating. The degree of formation of target neoantigen strongly depends on heating time and temperature. Quantity of target neoantigen depends also on milk normalization strategies, drying and condensation parameters. Guaranteed fresh cows milk, treated by modern sterilization technologies (eg UHT) show absolute absence of target neoantigen; it allows to determine very sensitively the addition of dry milk or crude technology violations and/or adulterations.

Kit performance data (see also IFU):
Test principle: sandwich, monoclonal antibodies
Total time of analysis: 30/30/15 min
Sensitivity: 0.001 U/ml
Material tested: liquid milk, dairy products

Estimation of antigen presence in different sources
Sample typeDry milk antigen U/mlComments
Control material
Fresh cow's milk<0.001Obtained during 3 hours after milking
Whole dry milk, 25% >2For food purposes
Normalized dry milk, 25%>20
Dry milk, ultra heat treated~100000For research use only
Clabbered milk<0.001Naturally, or by acid treatment
Tested random from local supermarket
Heated fresh milk50During 6 hours at 90 C
UHT fresh milk, normalized<0.001Labelled as dry milk FREE
Sterilized fresh milk, normalized<0.001

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