Toxoplasmosis is a widespread infection caused by the intracellular protozoan parazite Toxoplasma gondii. Pets (cats and dogs) may serve as intermediate host for T. gondii and transmit infection to humans. In most of cases, toxoplasmosis is a mild or asymptomatic disease; however, in immunocompromised patients this disease may be very severe and even life-threatening. Another risk group is pregnant women in whom primary toxoplasmosis can be transfected to the fetus, causing abortion, and severe malformations. Testing of cats and dogs for toxoplasmosis is used to detect carrier state and thus to reveal the risk for humans. >
100*
K
2
30//30/10-20'
37 °C
12
ÈÔÀ
604
0
K104CF
HSV 1/2 IgG-CF EIA
96
Herpes simplex virus (HSV) is one of the most common pathogens in mammals. HSV is transmitted by all secretions of infected body, especially via saliva, semen and cervical fluid. Latency and re-activation are characteristic for HSV infection. Specific IgG-antibodies to HSV are protective to some extent - they help to prevent the disease and limit latency, although do not provide a complete protection. A high titer of anti-HSV IgG-antibodies may suggest either a recent acute infection or reactivation of a latent one. Specific anti-viral treatment as well as non-specific activation of the immune system inhibit production of viral antigens and, therefore, induce decline of anti-HSV-IgG antibodies to their normal values. >
100*
K
2
30//30/10-20'
37 °C
12
ÈÔÀ
604
0
K105CF
Chlamydia IgG-CF EIA
96
Chlamydiosis is an infection disease affecting both animals and humans. Clinical manifestations may be the following: abortion, endometritis, vaginitis, mortinatality, nonviable offspring, encephalomyelitis, polyarthritis, conjunctivitis, pneumonia, enteritis, mastitis, orchitis, urethritis, balanoposthitis. A wide spread of infectious agents in natural surrounding (wild animals, especially – birds) represent a constant threat for humans. According to a new classification, Chlamydiaceae family is divided into 2 genera: Chlamydia and Clamydophila. Chlamydia include the following species: C. trachomatis (affects humans and laboratory animals), C. suis (affects pigs), C. muridarum (affects mice, hamsters). Chlamydophila include the following species: C. pneumoniae (affects humans and horses), C. pecorum (affects large horn cattle, sheep, goats, pigs), C. psittaci (affects birds, dogs and large horn cattle), and C. psittaci subspecies: C. abortus (affects large horn cattle, sheep, goats, horses, pigs, dogs), C. caviae (affects guinea pigs), C. felis (affects cats). Both genera of Chlamydiaceae share common antigens. Ca. 18-38% of dogs are infected with Chlamydia with clinical manifestations seen only sporadically. In cats, ca. 65-70% are Chlamydia carriers (mostly, asymptomatic) with living Chlamydia organisms found in saliva, urine, on pads. Various authors report ca. 5-10% cats having chronic chlamydiosis. Chlamydiosis is accompanied by a low production of neutralizing antibodies – they are produced in low titers and not constantly. Elevated IgG antibody titer indicates a persisting infection or recent reactivation.>
100*
K
2
30//30/10-20'
37 °C
12
ÈÔÀ
604
0
K106CF
Mycoplasma IgG-CF EIA
96
Mycoplasmae represent a separate class of microorganisms. Unique metabolic properties of Mycoplasmae determine their poor growth on standard microbiological media and require the application of serological methods in diagnostics. All Mycoplasmae share common antigenic epitopes; the microbes cause pneumonia, bronchitis and bullous meningitis; acute or chronic pelvic inflammations and may contribute to male and female infertility. Specific IgG-antibodies to Mycoplasmae do not possess protective properties; however their serum titer reflects the degree of microbial growth. Therefore, the detection of serum IgG antibodies may be used for disease and treatment monitoring. Elevated serum IgG antibody titers are detected after 3-4 weeks from the onset of the disease in spite of successful antibiotic treatment. >
100*
K
2
30//30/10-20'
37 °C
12
ÈÔÀ
604
0
K122C
Candida IgG-C EIA
96
Candidosis is a rare pathology affecting skin and mucous surfaces and caused by fungi of Candida species. Various Candida species are pathogenic, C. albicans and C. tropicalis being the most common. Alteration of normal microflora and/or immune status due to other pathology or drugs intake facilitates disease progression. The main symptoms are: erosive non-healing lesions covered by a greyish-white layer and surrounded by a red hem. Erosions are found on mucous surfaces of the mouth, throat, esophagus, on praeputium, in the anus and on skin-mucous borders. Various Candida species may also be the causative agents of erosive skin lesions, chronic and acute diarrhea, otitis externa, abscesses of interdigital folds, and inflammation of the nail bed. Substantial alteration of cellular immunity may lead to more serious pathologies, such as lesions of the intestine, gall bladder and ducti, bronchi and urinary tract as well as systemic candidosis (sepsis) with living Candida fungi found in circulation and Candida antigens detected in urine. Specific IgG-antibodies to Candida antigens are not protective (they do not protect animals from recurrent or repeated infection), but their titer usually reflects activity of fungal growth and therefore may be used to monitor such infections. Despite effective antifungal therapy, elevated serum levels of IgG-antibodies may be found 3-4 weeks after the onset of the disease. >
100*
K
2
30//30/10-20'
37 °C
12
ÈÔÀ
604
0
K151C
Parvo-IgG EIA
96
Parvovirus genus includes several specii, the canidae Parvovirus (CPV) type 2 being defined as a causative agent of parvoviral enteritis affecting Canidae and Mustelidae. CPV induces a rapid and serious disease with high mortality, especially in non-immunized animals. The symptoms are not specific to CPV infection and include chronic diarrhoea, dehydration, aphasia, vomiting and panleucopenia. In newborn puppies myocardial damage is also often seen. The female dog antibodies give protection to puppies until 5-6 weeks of age. The latter get their own immunological protection after week 8 only. Serum IgG antibodies to CPV appear on day 4-6 after infection with their maximal titre being reached on day 10-12. Anti-CPV IgG antibodies are protective. That is why vaccination is widely used to prevent infection. Quantitative determination of IgG-antibodies to CPV is used to estimate effectiveness of vaccination and to diagnose the infection in non-immunized animals. >
100*
5 U/ml
2
30//30/10-20'
37 °C
12
ÈÔÀ
711
0
K152C
CDV-IgG EIA
96
canidae/mustelidae Distermer disease is caused by a virus of the Pramyxoviridae family. Very contagious, it affects mainly young animals by direct contamination through the respiratory tract. The disease course may be divided into two stages: fever stage and nervous stage. Fever stage is comparatively easy to cure and rarely leads to death. Nervous stage develops if the animal is not cured and is characterized by damage of the CNS caused by autoimmune reactions. This stage usually ends with animal’s death. Among dogs, most sensitive to the disease are huskies, poodles, German sheepdogs and some other breeds. A part of dogs (ca. 20%) are genetically deficient for anti-CDV immune response, such animals being very reluctant to treatment and usually dying. CDV-IgG plays a protective role – that is why prophylaxis of this infection is based on vaccination. This test may be used both to estimate effectiveness of vaccination and to detect infection in non-immune animals. >
100*
10 U/ml
2
30//30/10-20'
37 °C
12
ÈÔÀ
711
0
K207CF
Progesterone-CF EIA
96
Progesterone is a gestagen with a MW of 314.5 Dalton. Progesterone is secreted by corpus luteum, adrenals and testis; it plays a role of a precursor for corticosteroids and androgens. Being an estrogen antagonist, Progesteron induces characteristic changes in endometrium necessary for implantation of an impregnated ovum. >
50
0,5 nmol/l
1
//120/10-20'
37 °C
12
ÈÔÀ
606
0
K207M
Progesterone milk EIA
96
Progesterone is a gestagen with a MW of 314.5 Dalton. Progesterone is secreted by corpus luteum, adrenals and testis; it plays a role of a precursor for corticosteroids and androgens. Being an estrogen antagonist, Progesteron induces characteristic changes in endometrium necessary for implantation of an impregnated ovum. During pregnancy, Progesterone concentration continuously increases at least by week 10-12, and it induces proliferation and development of mammary glands and inhibits ovulation. A certain proportion of serum Progesterone penetrates into milk; this enables to detect pregnancy in cows using non-invasive sample collection method. >
