Immunoassays for detection of Phoma: development and applications

Phoma genus are mostly saprophytic or pathogenic (causative agents for phomosis) of fruits, vegetables, grasses and cereals. This genus also plays an important role in biodestruction of organics and even synthetic material (plastics). Rarely, Phoma may also cause infections and allergy in animals and humans

We have grown different speciei of Phoma
Phoma betae (Ag825) extract was used as the source of immunogen to immunize rabbits.

Rabbit antiserum against Phoma (AS825) was further enriched by affinity chromatography on immobilized antigen. Affinity purified antibodies (AS825 A) are available for research.

Affinity antibody against Phoma was used for immunocytochemical staining of fungal culture.
The pictures (see below) confirm the localization of target antigen in conidia.

Control staining (no anti-Phoma antibodies)

Specific staining (anti-Phoma antibodies)

ELISA kit (K825) was constructed from AS825 antiserum as single site inhibitory assay, see IFU

Accumulation of conidial antigens measured by K825 Phoma EIA in cultivation medium correlates well with the number of conidia (see figure below)